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目的分析单核细胞膜表面外周血CD14+单核细胞HLA-DR及CD14+在肺炎患儿实施治疗前后表达的改变情况。方法按病情严重程度将2013年1月—2014年1月收治的60例肺炎患儿分为轻型肺炎组(35例)和重型肺炎组(25例)。于治疗前后使用流式细胞仪对两组患儿单核细胞膜表面HLA-DR及CD14+进行检测,计量资料采用t检验;计数资料进行χ2检验,P<0.05为差异具有统计学意义。结果治疗前,轻型肺炎组CD14+表达率为(5.38±2.29)%,HLA-DR表达率为(66.47±10.35)%;治疗后该组CD14+表达率为(4.83±1.62)%,HLA-DR表达率为(70.25±9.72)%。治疗前重型肺炎组CD14+表达率为(1.82±1.52)%,HLA-DR表达率为(46.52±11.35)%;治疗后该组CD14+表达率为(4.83±1.59)%,HLA-DR表达率为(69.62±6.95)%。与治疗前相比,两组患儿外周血单核细胞表面抗原HLA-DR表达率均明显升高,差异具有统计学意义(P<0.05)。重型肺炎组治疗前HLA-DR及CD14+表达率均低于轻型肺炎组,差异具有统计学意义(P<0.05)。结论 CD14+与HLA-DR可作为反映肺炎患儿病情严重程度的重要指标,具有一定的辅助诊断价值。
Objective To analyze the expression changes of HLA-DR and CD14 + CD14 + monocytes in peripheral blood mononuclear cells before and after the treatment of pneumonia. Methods Sixty children with pneumonia admitted from January 2013 to January 2014 were divided into mild pneumonia group (n = 35) and severe pneumonia group (n = 25) according to the severity of the disease. Flow cytometry was used to detect the expression of HLA-DR and CD14 + on the mononuclear cell membrane in both groups before and after treatment. The measurement data were analyzed by t-test. The count data were analyzed byχ2 test. P <0.05 was considered statistically significant. Results Before treatment, the expression of CD14 + in mild pneumonia group was (5.38 ± 2.29)% and that of HLA-DR group was (66.47 ± 10.35)%, and the positive rate of CD14 + expression was (4.83 ± 1.62)% The rate was (70.25 ± 9.72)%. The expression of CD14 + in severe pneumonia group before treatment was (1.82 ± 1.52)% and that of HLA-DR was (46.52 ± 11.35)%, and the positive rate of CD14 + expression was (4.83 ± 1.59)% (69.62 ± 6.95)%. Compared with those before treatment, the expression of HLA-DR in peripheral blood mononuclear cells in both groups were significantly increased, the difference was statistically significant (P <0.05). The levels of HLA-DR and CD14 + in severe pneumonia group before treatment were lower than those in mild pneumonia group, the difference was statistically significant (P <0.05). Conclusion CD14 + and HLA-DR can be used as an important index to reflect the severity of children with pneumonia, and has some auxiliary diagnostic value.