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目的:比较厚朴不同粒径粉体中主要活性成分厚朴酚、和厚朴酚溶出情况,为厚朴超微粉体的粒径控制与制剂生产提供实验依据。方法:采用高效液相色谱法分别对厚朴超微粉体和普通粉体中所含主要活性成分的提取率及不同溶出介质的相对累积溶出率进行定量研究。色谱条件:采用ZORBX-C18(4.6 mm×150 mm,5μm)色谱柱,流动相为甲醇-水(75∶25),流速1 m L·min~(-1),检测波长294 nm,柱温30℃,进样量10μL。结果:厚朴超声提取的厚朴酚及和厚朴酚的相对提取率较回流提取高,且不同粒度粉体的相对提取率大小为60 Hz超微粉体>23 Hz超微粉体>42 Hz超微粉>常规粉体,厚朴不同粒度粉体在不同溶出介质中的相对累积溶出率大小为人工胃液>蒸馏水>人工肠液;在本次试验所选4个粒度粉体中,60 Hz超微粉体为最佳粒度粉体。厚朴超微粉体与普通粉体主要活性成分的提取率以及在水、人工胃液、人工肠液中的相对累积溶出率差异显著。结论:适度的微粉化能促进厚朴有效成分的溶出,其溶出行为还受到不同溶出介质的影响,超微粉碎技术应用于厚朴药材具有可行性。
OBJECTIVE: To compare the dissolution of honokiol and honokiol, the main active components of Magnaporthe grosvenori, with different particle sizes, and provide the experimental basis for the particle size control and preparation production of Magnaporthe giganteum powder. Methods: The HPLC method was used to quantitatively study the extraction rate of the main active ingredients contained in the ultrafine powder and ordinary powder, and the relative cumulative dissolution rate of different dissolution media. Chromatographic conditions: The mobile phase was methanol-water (75:25) at a flow rate of 1 mL · L -1 at a wavelength of 294 nm using a ZORBX-C18 column (4.6 mm × 150 mm, 5 μm) 30 ℃, injection volume 10μL. Results: The relative extraction rates of honokiol and honokiol extracted by Magnolia bark were higher than that of reflux extract, and the relative extraction rate of different size powders was 60 Hz. Micropowder> 23 Hz Micropowder> 42 Hz ultrafine powder> The relative cumulative dissolution rate of conventional powder and Magnolia different particle size powders in different dissolution media was artificial gastric juice> distilled water> artificial intestinal juice. Among the four particle sizes selected in this experiment, Smell powder for the best size. The extraction rate of the main active components of the superfine powder and ordinary powder of Magnaporthe grisea and the relative cumulative dissolution rates in water, artificial gastric juice and artificial intestinal juice were significantly different. Conclusion: Appropriate micronization can promote the dissolution of active ingredients of Magnolia officinalis. The dissolution behavior of Magnolia officinalis is also influenced by different dissolution media. It is feasible to apply the micronization technique to Magnolia officinalis.