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[Objective] The paper was to establish the identification method of Coptis chinensis and Forsythia suspensa in Qingwen baidu granules. [Method]With benzene-ethyl acetate-methanol-isopropanol-concentrated ammonium liquid( 12∶6∶3∶3∶1) as the developer,the samples were outspread in the cylinder with saturated ammonia steam by thin layer chromatography( TLC),and the existence of C. chinensis and berberine hydrochloride was checked under UV lamp. With trichloromethane-methanol( 5∶1) as the developer and 10% sulfuric acid ethanol solution as the chromogenic reagent,the samples were heated at 105 ℃ for 5 min to check the existence of F. suspense under natural light. [Result]Qingwen baidu granules had the same spots with C. chinensis control and berberine hydrochloride at the same Rf value under 365 nm UV lamp; Qingwen baidu granules had the same spots with F. suspensa control at the same Rf value under natural light. [Conclusion] A TLC method detecting C. chinensis and F. suspensa in Qingwen baidu granules was established,which had good specificity and repeatability,suitable for rapid detection of C. chinensis and F. suspensa. It could be well applied to control the quality of Qingwen baidu granule.
[Objective] The paper was to establish the identification method of Coptis chinensis and Forsythia suspensa in Qingwen baidu granules. [Method] With benzene-ethyl acetate-methanol-isopropanol-concentrated ammonium liquid (12:6: 3: 3: 1) as the developer, the samples were outspread in the cylinder with saturated ammonia steam by thin layer chromatography (TLC), and the existence of C. chinensis and berberine hydrochloride was checked under UV lamp. With trichloromethane-methanol (5: 1) as the developer and 10% sulfuric acid ethanol solution as the chromogenic reagent, the samples were heated at 105 ℃ for 5 min to check the existence of F. suspense under natural light. [Result] Qingwen baidu granules had the same spots with C. chinensis control and berberine hydrochloride at the same Rf value under 365 nm UV lamp; Qingwen baidu granules had the same spots with F. suspensa control at the same Rf value under natural light. [Conclusion] A TLC method detecting C. chinensis and F. suspensa in Qingwen baidu granules was established, which had good specificity and repeatability, suitable for rapid detection of C. chinensis and F. suspensa. It could be well applied to control the quality of Qingwen baidu granule.