论文部分内容阅读
研究北虫草超氧化物歧化酶(SOD)的分离纯化和部分酶学特性。结果表明,从北虫草中提取SOD的最佳工艺条件为原料与PBS(pH7.73)配比为1∶3(m∶v),提取时间为3h,纯化倍数可达到粗酶液的4.09倍。提纯后的SOD在690nm左右出现了特征吸收峰,最适温度38℃,最适pH值为7.8,大部分糖对SOD的活性没有显著影响,有机酸都有降低酶活性的作用,过氧化氢对该酶有抑制作用,北虫草SOD是以铜和锌离子为辅基,为北虫草的进一步开发和利用提供了理论依据。
To study the isolation, purification and partial enzymatic properties of Cordyceps militaris superoxide dismutase (SOD). The results showed that the optimal extraction conditions for SOD from Cordyceps militaris were as follows: the ratio of raw material to PBS (pH7.73) was 1: 3 (m: v), the extraction time was 3h and the purification times could reach 4.09 times . The purified SOD showed a characteristic absorption peak around 690 nm, the optimum temperature was 38 ℃, and the optimum pH was 7.8. Most of the sugars had no significant effect on the activity of SOD. Organic acids all had the effect of reducing the activity of enzyme. Hydrogen peroxide The inhibition of the enzyme, Cordyceps SOD is a copper and zinc ions as a cofactor for the further development and utilization of Cordyceps provides a theoretical basis.