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为进一步研究共生菌在褐飞虱对吡虫啉产生抗性中的生理生化机制,在稻田杀虫剂对褐飞虱共生解脂假丝酵母生长影响的基础上,选用不同吡虫啉浓度进行抗药性菌株的驯化。结果表明,褐飞虱共生解脂假丝酵母在不同吡虫啉浓度(2 000、1 000和500 mg/L)的固体培养基上继代培养,经过20代后2 000 mg/L培养基上的共生菌菌落数量,与未加吡虫啉的培养基上的菌落数量差异不明显,并且连续3代稳定后定为抗2 000 mg/L吡虫啉的共生菌菌株。在光镜下比较不同抗感吡虫啉菌株假菌丝的形态变化,发现抗吡虫啉菌株的假菌丝出现畸形,而且假丝变短,部分出现了膨大。
In order to further study the physiological and biochemical mechanisms of commensal bacteria resistance to imidacloprid in N. lugens, based on the effects of rice insecticides on the growth of N. lugens Symbiotic Candida albicans, different concentrations of imidacloprid were chosen to domesticate resistant strains. The results showed that the Candida albicans symbiosis Candida parahaemolyticus was subcultured on solid media with different imidacloprid concentrations (2000, 000 and 500 mg / L), and after 20 generations the symbiotic bacteria There was no significant difference between the number of colonies and the number of colonies on the medium without imidacloprid, and the symbiotic strains resistant to 2 000 mg / L imidacloprid after 3 consecutive generations were stable. Under light microscope, the morphological changes of pseudomycoplasma were compared with different anti-infective imidacloprid strains. It was found that the pseudomycosis of the anti-imidacloprid strain appeared deformity, and the false-cut silk became short and partially enlarged.