论文部分内容阅读
目的:探讨重度子痫前期患者外周血中NK细胞数量、杀伤功能以及相关细胞因子的变化。方法:选择重度子痫前期患者25例(研究组)以及正常妊娠妇女25例(对照组),流式细胞术测定外周血中NK细胞数量,细胞毒实验测定NK细胞的杀伤活性,ELISA法测定血清IFN-γ及IL-2的浓度。结果:研究组患者外周血中NK细胞的数量以及杀伤活性显著高于对照组;研究组外周血中IFN-γ、IL-2的浓度也显著高于对照组。结论:重度子痫前期患者外周血NK细胞数量增多,杀伤活性增强,血清中NK细胞相关细胞因子也增加。这些改变可能参与重度子痫前期的发病机制。
Objective: To investigate the changes of NK cell number, cytotoxicity and related cytokines in peripheral blood of patients with severe preeclampsia. Methods: 25 cases of severe preeclampsia (study group) and 25 cases of normal pregnant women (control group) were selected. The number of NK cells in peripheral blood was determined by flow cytometry. The cytotoxic activity of NK cells was measured by cytotoxicity assay. Serum levels of IFN-γ and IL-2. Results: The number of NK cells in the peripheral blood and the cytotoxicity of the study group were significantly higher than those in the control group. The concentrations of IFN-γ and IL-2 in the study group were also significantly higher than those in the control group. Conclusion: The number of NK cells in peripheral blood of severe preeclampsia increased, and the cytotoxicity of NK cells in serum increased. These changes may be involved in the pathogenesis of severe preeclampsia.