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目的探讨丙型肝炎病毒（ＨＣＶ）基因重组体诱发小鼠免疫反应。方法将编码Ⅱ型ＨＣＶ结构蛋白的基因片段Ｃ、Ｅ１和大部分Ｅ２插入到真核细胞表达载体ｐＣＤＳＲα１中，构建成重组体ｐＣＤＨＣＶ１。经肌内注射将此重组体免疫Ｂａｌｂ／ｃ小鼠。结果ｐＣＤＨＣＶ１（１００μｇ／只，ｎ＝１２）３～４次接种小鼠后，血清抗体水平达０．７１～０．７７（Ａ值，下同），空载体ｐＣＤＳＲα１免疫鼠（ｎ＝８），血清抗体阴性；对其中８只免疫鼠持续检测１８周，未见抗体水平有下降趋势（０．６８～０．７５）。重组体ｐＣＤＨＣＶ１免疫鼠（ｎ＝１２）的脾细胞对ＨＣＶ合成肽ＣＰ９、基因重组抗原Ｃ、Ｅ１刺激后，均出现增殖反应（ｃｐｍ），ＳＩ分别为４．１４±０．９，３．９８±１．６，４．０２±１．２，与ｐＣＤＳＲα１免疫鼠（ｎ＝８）相比，差异有显著性（Ｐ＜０．００１）。结论构建的ＨＣＶＤＮＡ重组体可诱发Ｂａｌｂ／ｃ小鼠产生免疫反应。
Objective To investigate the immune response induced by recombinant hepatitis C virus (HCV) gene in mice. Methods The gene fragment encoding type Ⅱ HCV structural protein C, E1 and most E2 were inserted into eukaryotic expression vector pCDSRα1 and constructed into recombinant pCDHCV1. This recombinant was immunized Balb / c mice by intramuscular injection. Results The antibody levels of pCDHCV1 (100μg / mouse, n = 12) were 0.71 ~ 0.77 (A value, the same below) = 8). Serum antibodies were negative. Eight of the eight immunized mice were continuously tested for 18 weeks. No decrease in antibody level (0.68-0.75) was observed. The splenocytes from recombinant pCDHCV1 immunized mice (n = 12) exhibited proliferative response (cpm) to HCV synthetic peptide CP9 and gene recombinant antigen C and E1 with SIs of 4.14 ± 0.9 and 3 .98 ± 1.6 and 4.02 ± 1.2. There was a significant difference (P <0.001) between pCDSRα1 immunized mice and n = 8 mice. Conclusion The constructed HCVDNA recombinant can induce immune response in Balb / c mice.