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利用解剖法采集太平洋牡蛎精液 ,用自然海水做基础溶液 ,配制不同浓度的 DMSO(二甲亚砜 )作为超低温保护剂 ,在液氮 (L N2 )面上不同高度进行预处理 ,投入 LN2 内保存 ,升温解冻后 ,检测精子成活率。结果表明 ,DMSO处理浓度、预处理温度及时间和升温方式对超低温保存成活率均具有显著影响。其中 ,用 10 % DMSO在 LN2 面上 17cm预处理 6 min,采用 16～ 17℃流水不完全解冻 ,效果最好 ,超低温保存精子成活率可达 70 %。与新鲜精子相比 ,在 L N2 内保存一天的精子 ,授精能力没有明显变化。
Anatomical method was used to collect Pacific oyster semen, and the natural seawater was used as the basic solution. Different concentrations of DMSO (dimethylsulfoxide) were prepared as cryoprotectants and pretreated at different heights on liquid nitrogen (LN2) surface and stored in LN2 , After thawing, detect sperm survival rate. The results showed that the concentration of DMSO, the temperature and time of pretreatment and the method of temperature increase had a significant effect on the survival rate of cryopreservation. Among them, pretreatment with 10% DMSO for 17 min on LN2 surface for 6 min, and 16-17 ° C water did not completely thaw, the effect was best, and the survival rate of cryopreserved sperm was 70%. Compared with fresh sperm, the sperm stored in L N2 for one day did not change significantly in insemination ability.