雄黄(Realgar,As4S4)是一种含砷的硫化物,作为一种中药砷剂已有上千年的历史。近年来,雄黄治疗血液系统疾病、恶性淋巴系统疾病取得了明显的疗效,引起人们广泛的关注[1]。雄黄的传统制备方法包括酸提、煅烧、膜分离,以及机械研磨等[2-3],然而这些方法炮制的雄黄水溶性低、胃肠道吸收差、毒性高以及生物利用度低的缺点严重限制了它的临床应用[4]。生物浸出(Bioleaching)是一种从低品位矿物中提取金属 Realgar (As4S4) is an arsenic-containing sulfide that has been used as a traditional Chinese arsenic for thousands of years. In recent years, realgar treatment of hematological diseases, malignant lymphatic system has achieved significant efficacy, causing widespread concern [1]. The real preparation methods of realgar include acid extraction, calcination, membrane separation and mechanical grinding [2-3]. However, the realgar produced by these methods has low water solubility, poor gastrointestinal absorption, high toxicity and low bioavailability, Limit its clinical application [4]. Bioleaching is the extraction of metals from low-grade minerals