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通过对具有不同种植年限和不同种植强度的转Bt基因水稻与非转Bt基因水稻土壤中的细菌以及固氮细菌群落结构进行研究,发现转Bt水稻的种植可能会影响土壤中微生物群落的多样性,但是这种影响的可能只是暂时的,通过对测量种植水稻的芽长实验也得出相似的结论.另外,根据16S rDNA基因构建的系统发育进化树揭示了本实验分离的固氮细菌的遗传多样性,发现实验土壤中的固氮细菌主要分为放线菌门(Actinobacteria)(92%)和α-变形菌门(α-Pro-teobacteria)两大类.分离出的8株典型的固氮菌株,其16S rDNA基因和固氮基因nifH的序列两者的分布不一致,nifH的分布更为紧凑,为固氮基因可能发生了原位水平基因转移提供了证据.
By studying the bacteria and the structure of nitrogen-fixing bacteria in transgenic Bt rice and non-transgenic Bt rice soil with different planting years and planting intensities, we found that the Bt rice planting may affect the diversity of soil microbial communities, However, this effect may only be temporary, and similar conclusions have been drawn from the sprout experiments on measuring rice planting.In addition, phylogenetic tree based on 16S rDNA gene revealed the genetic diversity of nitrogen-fixing bacteria isolated from this experiment , And found that the nitrogen-fixing bacteria in the experimental soil were mainly divided into two categories: Actinobacteria (92%) and α-Pro-teobacteria.The eight typical nitrogen-fixing bacteria The distribution of both the 16S rDNA gene and the nitrogen-fixing gene nifH is not consistent, and the distribution of nifH is more compact, which provides evidence that the nitrogen-fixing gene may have horizontal gene transfer in situ.