论文部分内容阅读
经1996年至1998年室内接种试验,分别比较了人工饲养带毒和自然带毒的茶翅蝽成虫和若虫的传病特点。结果显示:室内人工饲养带毒的3龄、4龄若虫接种泡桐发病率分别达61.7% 和46.5% ,高于自然带毒的若虫16% 的传病率。室内饲养带毒成虫的接种发病率为14.1% ,高于自然带毒成虫4.5% 的传病率。寄主体内病原的最短潜育期(MIP)室内饲毒3龄若虫为34 d,4龄为45 d,成虫处理为219 d;自然带毒若虫为243 d,成虫处理为257 d。6月接种苗多在当年表现病状,7月接种苗均在次年发病。3龄、4龄若虫接种后病原潜育期(IP)与泡桐苗累计发病率的关系分别为y= 0.847 2x+ 23.044(R2= 0.874 3),y= 0.759x+ 29.022 (R2= 0.801 9);由此得到相应的3龄潜育期中值LP50= 65.40 d 和4龄LP50= 66.472 d。室内3龄、4龄若虫在充分吸食病组织后,其传病力差异不大,且传毒后病原在寄主体内的潜育期中值没有明显的差异。
After 1996 to 1998 indoor vaccination test, respectively, compared with artificial feeding poisonous and naturally poisoned Entomophthora larvae and nymph transmission characteristics. The results showed that the incidence rates of paulownia inoculated with artificial feeding 3rd instar and 4th instar nymphs were 61.7% and 46.5% respectively, which were higher than those of naturally infected nymphs. The incidence of indoor incubation with poisoned adults was 14.1%, which was higher than the 4.5% prevalence of naturally infected adults. The minimum latent period (MIP) of pathogen in the host was 34 days for the third instar nymphs, 45 days for the fourth instar and 219 days for the adult worms. The natural nymphs were 243 days and the adults were 257 days. Inoculated seedlings in June more than the performance of symptoms in the year, July vaccination are in the following year onset. The relationship between the pathogenicity (IP) of the 3rd instar and the 4th instar nymphs after vaccination and the cumulative incidence of Paulownia seedlings were y = 0.847 2x + 23.044 (R2 = 0.874 3) and y = 0.759x + 29 respectively. 022 (R2 = 0.801 9). The corresponding median LP50 = 65.40 d in the third instar larvae and LP50 = 66.472 d in the fourth instar larvae were obtained. There was no significant difference in the pathogenicity of 3rd instar and 4th instar nymphs after full ingestion of the diseased tissues, and there was no significant difference in the median of the latent period of the pathogen in the host after being infected.