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目的探讨导流杂交基因芯片技术作为临床检测人乳头瘤病毒(HPV)常规方法的实用性,及在宫颈癌筛查中的临床应用价值。方法采用导流杂交基因芯片技术对676例疑似HPV感染的标本进行基因诊断和分型,检测了13种高危亚型(16,18,31,33,35,39,45,51,52,56,58,59和68型)、5种低危亚型(6,11,42,43和44型)和3种中国人群常见亚型(53,66和CP8304型)。结果 676例样本中检出HPV阳性175例,检出率25.9%,高危占74.9%、低危占16.0%、中国人常见亚型占9.0%、2种或2种以上亚型混合感染占15.4%。结论导流杂交基因芯片技术适合于临床筛查HPV感染,能更好的检出HPV感染的基因型,为临床提供较有价值的实验资料,对宫颈癌的筛查有较大的帮助。
Objective To investigate the practicability of flow-through hybridization gene chip technique as a routine method for clinical detection of human papillomavirus (HPV) and its clinical value in cervical cancer screening. Methods Six hundred and sixty-six human papillomavirus (HPV) -positive specimens were genotyped and diagnosed by flow-through hybridization gene chip technique. Thirteen high-risk subtypes (16,18,31,33,35,39,45,51,52,56 , 58, 59 and 68), five low-risk subtypes (6, 11, 42, 43 and 44) and three common subtypes (53, 66 and CP8304) of the Chinese population. Results A total of 175 HPV positive cases were detected in 676 samples, with a detection rate of 25.9%, a high risk of 74.9%, a low risk of 16.0%, a Chinese common subtype of 9.0%, and two or more subtypes of mixed infections accounting for 15.4% %. Conclusion Diversified flow-through hybridization gene chip technology is suitable for clinical screening of HPV infection and better detection of genotypes of HPV infection, providing more valuable experimental data for clinical diagnosis of cervical cancer is of great help.