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七十年代相继在重症肌无力(MG)实验动物模型和患者的血清中检出抗乙酰胆硷受体(AChR)的抗体(AChRab),阐明此抗体与MG的发病密切相关。嗣后,建立了多种微量检测方法,并对AChRab与MG的临床分型、胸腺组织学、人类白细胞抗原(HLA)及各种治疗等因素的关系作了深入探讨。本文就近年来有关研究的概况作一略述。 AChRab的定量检测 (一)检测方法计有毒素结合抑制、双抗体免疫沉淀、补体结合、免疫萤光及酶联免疫吸附等方法。以双抗体免疫沉淀(双抗)法最为常用,其测定基本原理为:用去污剂Triton X-100提取的骨骼肌AChR能特异地与~(125)碘-α-银环蛇毒素(~(125)I-α-BGT)及
In the seventies, anti-acetylcholine receptor (AChRab) antibody (AChRab) was detected in sera of patients with myasthenia gravis (MG) and in the serum of patients, which showed that this antibody was closely related to the pathogenesis of MG. Subsequently, a variety of trace detection methods were established and the relationship between AChRab and clinical classification of MG, thymus histology, human leukocyte antigen (HLA) and various treatment factors were discussed in depth. This article gives a brief overview of the research in recent years. Quantitative detection of AChRab (a) detection methods count toxin binding inhibition, double antibody immunoprecipitation, complement fixation, immunofluorescence and enzyme-linked immunosorbent assay. The dual-antibody immunoprecipitation (double-antibody) method is most commonly used. The basic principle of the assay is that the AChR of skeletal muscle extracted with the detergent Triton X-100 can specifically react with ~ (125) iodine- (125) I-α-BGT) and