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目的联合细胞形态学、免疫分型检测手段探讨染色体分析在急性白血病AL诊断及预后判断中的价值。方法 310例AL患者于治疗前采用骨髓直接法和(或)短期培养法,按常规进行染色体制备,用G显带技术分析中期细胞数10-30个,平均每例分析细胞20个,核型按《人类细胞遗传学国际命名法体制(ISCN)1995》进行描述。结果急性髓系白血病(AML)异常核型中,以t(8;21)和M2最多见,其次是t(15;17)和M3、inv(16)和M4Eo、t/del(11q23)和M5等。急性淋巴细胞系白血病(ALL)患者中异常检出率最高的是t(9;22),数目异常以超二倍体、多倍体常见。结论联合细胞形态学、免疫表型分析、染色体检查技术对于白血病的准确诊断、靶向治疗、判断预后具有重要意义。
Objective To investigate the value of chromosome analysis in the diagnosis and prognosis of acute leukemia with AL by combining with cell morphology and immunophenotyping. Methods A total of 310 patients with AL were treated with the direct method of bone marrow and / or short-term culture before the treatment. Chromosome preparation was performed routinely. The number of metaphase cells was analyzed by G-banding technique. The average number of cells was 20 in each case. Karyotype According to the “International Nomenclature System of Human Cytogenetics (ISCN) 1995” to describe. Results Aneurysm of acute myeloid leukemia (AML) was found with the highest number of t (8; 21) and M2, followed by t (15; 17) and M3, inv (16) and M4Eo, t / del M5 and so on. Acute lymphoblastic leukemia (ALL) in patients with the highest detection rate of abnormal t (9; 22), the number of abnormal hyperdiploid, polyploid common. Conclusion The combination of cell morphology, immunophenotyping and chromosomal examination is of great significance for the accurate diagnosis, targeted therapy and prognosis of leukemia.