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胎儿出生以后,造血干细胞(HSC)才从胎儿的肝脏和脾脏转移到骨髓,这一过程可能由不同的造血微环境中的信号分子所介导。间充质干细胞(MSC)是骨髓微环境中间质细胞如成骨细胞、内皮细胞的前体祖细胞。研究者推测,胎儿出生前的骨髓可能并不特别适合HSC生长。然而,该假说尚缺乏直接的证据支持。本研究通过对胎儿和成人骨髓MSC的造血支持能力进行比较,拟为此提供证据。成人骨髓MSC来源于3位健康供者,胎儿骨髓MSC来源于孕19-20周流产的胎儿。MSC辐照后与CD34+一起进行长期培养启动细胞分析,计数克隆形成细胞的数量,流式分析培养后CD34+的表型变化。RT-PCR分析两种MSC中细胞因子的表达。结果显示,成人骨髓MSC比胎儿骨髓MSC具有更强的造血支持能力,两者都促进CD34+向髓系细胞分化,两者之间细胞因子的表达存在差异。结论:与胎儿骨髓MSC相比,成人骨髓MSC在某些治疗,尤其是促进造血恢复方面具有更广泛的应用前景。
After the baby is born, hematopoietic stem cells (HSCs) are transferred from the liver and spleen of the fetus to the bone marrow, and this process may be mediated by signaling molecules in different hematopoietic microenvironments. Mesenchymal stem cells (MSCs) are progenitor cells of the stromal cells in the bone marrow microenvironment such as osteoblasts and endothelial cells. The researchers speculated that the prenatal bone marrow may not be particularly suitable for HSC growth. However, this hypothesis lacks direct evidence support. This study provides evidence by comparing the hematopoietic support capacity of fetal and adult bone marrow MSCs. Adult Bone Marrow MSCs originate from 3 healthy donors. Fetal bone marrow MSCs originate from fetuses of 19-20 weeks of abortion. After irradiation, the CD34 + cells were incubated with CD34 + for long-term culture to initiate cell counting. The number of clonogenic cells was counted and the CD34 + phenotypes were analyzed by flow cytometry. The expression of cytokines in both MSC were analyzed by RT-PCR. The results showed that adult bone marrow MSCs had stronger ability of hematopoietic support than fetal bone marrow MSCs, both of which promoted the differentiation of CD34 + to myeloid cells, and there were differences in the expression of cytokines between the two. Conclusion: Compared with fetal bone marrow MSCs, adult bone marrow MSCs have more extensive application prospects in some treatment, especially for hematopoietic recovery.