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基因工程是解决昆虫病原真菌致死慢、致死率低的有效途径,但在传代培养过程中,被转入的基因是否会丢失以及转基因工程株是否会像野生型菌株那样频繁地发生变异,引起产孢减少和毒力减退等生产性状的退化?针对这些问题,研究一株球孢白僵菌Beauveria bassiana转外源蝎毒基因工程菌株在继代培养过程中的的变异现象,发现工程株也和野生型菌种一样会发生菌落局变,但所插入的蝎毒基因并未丢失。与野生型出发菌株及其单孢分离株相比,工程株的角变率居高不下而产孢量及对松毛虫的毒力显著降低。然而,从该工程株筛选出的一株单孢分离株在继代培养中却比较稳定,产孢量及对松毛虫的毒力则远高于野生型出发菌株及其单孢分离株,是一株稳定的高产高毒优良菌株。
Genetic engineering is an effective way to solve the problem of slow death and low lethality of entomopathogenic fungi. However, in the process of subculture, whether the gene will be transferred will be lost and whether the transgenic engineering strains will mutate frequently like the wild type strains, Spore reduction and virulence decline and other traits of degradation? In response to these problems, the Beauveria bassiana Beauveria bassiana transgenic exogenous scorpion venom genetically engineered strains in the subculture of the mutation phenomenon found that engineering strains and Colony changes occur in wild-type strains, but the inserted scorpion venom gene is not lost. Compared with wild-type starter strains and single-spore isolates, the strain rate of the engineering strains was high while the sporulation amount and the virulence of Dendrolimus punctatus were significantly reduced. However, a strain isolated from this engineering strain was stable in subculture, its sporulation capacity and its toxicity to pine caterpillars were much higher than those of wild-type and single-spore isolates A stable strain of high yield high-toxic strains.