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目的:探讨中成药醒脑静注射液对老年麻醉后认知功能障碍大鼠学习记忆功能及海马区一氧化氮合酶(n NOS)、血清β-淀粉样蛋白的影响,研究其改善老年认知障碍大鼠学习记忆功能的可能机制。方法:将40只老年大鼠随机分为空白对照组10只,余30只制备老年认知障碍大鼠模型后随机分为模型对照组10只、醒脑静低剂量组10只、醒脑静高剂量组10只,共4组。采用Morris水迷宫观察并评价其对空间学习记忆的改善。免疫组化法观察海马组织一氧化氮合酶(n NOS)表达,采用酶联免疫吸附(ELISA)法对各组大鼠血清中β-淀粉样蛋白含量进行检测。结果:与空白对照组比较,模型对照组、醒脑静低剂量组、醒脑静高剂量组逃避潜伏期显著延长、穿越平台次数显著减少(P<0.01),给药6 d后实验组与模型对照组比较,醒脑静低剂量组、醒脑静高剂量组避潜伏期显著缩短,穿越平台次数显著增加(P<0.05,P<0.01)。与空白对照组比较,模型对照组、醒脑静低剂量组、醒脑静高剂量组海马组织一氧化氮合酶(n NOS)表达显著减少、血清β-淀粉样蛋白含量增多(P<0.01)。经治疗后,与模型组比较,醒脑静低剂量组、醒脑静高剂量组海马组织一氧化氮合酶(n NOS)表达显著增多,血清β-淀粉样蛋白含量显著减少(P<0.05,P<0.01)。结论:醒脑静注射液可增加海马区n NOS表达、降低血清β-淀粉样蛋白含量,从而保护海马组织形态的完整性,改善老年认知功能障碍大鼠的空间学习记忆能力。
Objective: To investigate the effects of Xingnao Jing injection on cognitive learning and memory function, nitric oxide synthase (nNOS) and β-amyloid in hippocampus of aged rats with cognitive impairment after anesthesia, Possible mechanism of learning and memory in rats with cognitive impairment. Methods: Forty aged rats were randomly divided into blank control group (n = 10), and the remaining 30 rats were randomly divided into model control group (n = 10), Xingnaojing low dose group (n = 10) High-dose group of 10, a total of 4 groups. Morris water maze was used to observe and evaluate the improvement of spatial learning and memory. The expression of nitric oxide synthase (nNOS) in hippocampus was observed by immunohistochemistry. The content of β-amyloid in sera of rats in each group was detected by enzyme linked immunosorbent assay (ELISA). Results: Compared with the blank control group, the escape latency of the model control group, Xingnaojing low dose group and Xingnaojing high dose group were significantly prolonged and the number of crossing the platform significantly decreased (P <0.01). Compared with the blank control group, The control group, Xingnaojing low dose group, Xingnaojing high dose group significantly shortened incubation period, the number of platforms across the platform increased significantly (P <0.05, P <0.01). Compared with the blank control group, nNOS expression in hippocampus of model control group, Xingnaojing low dose group and Xingnaojing high dose group were significantly decreased, and serum β-amyloid content was increased (P <0.01) ). After treatment, compared with the model group, the expression of nNOS and the content of β-amyloid in hippocampus of high dose Xingnaojing high dose group and Xingnaojing high dose group were significantly decreased (P <0.05 , P <0.01). Conclusion: Xingnaojing Injection can increase the nNOS expression in hippocampus and decrease the content of β-amyloid in the hippocampus, thus protecting the integrity of the hippocampal formation and improving the spatial learning and memory of the aged cognitive impairment rats.