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用两株具有鼻咽癌相关抗原内影像的抗独特型单克隆抗体2H4,5D3,经氢氧化铝凝胶沉淀法制备成抗独特型疫苗Alum-2H4,Alum-5D3,对19例晚期鼻咽癌放疗病人作主动免疫治疗,9例放疗加生理盐水注射为对照组.用ELISA检测治疗前后病人血清抗体和细胞因子水平.用原位Northem杂交检测外周血单个核细胞(PBMC)IL-2mRNA的表达.发现接受Alum-2H4或Alum-5D3治疗的病人无1例有过敏或其他毒副反应,血清中均检测到抗抗独特型抗体(Ab3)、抗肿瘤抗体(Ab1’)水平均有不同程度的增高,但也产生了人抗鼠抗体(HAMA).血清细胞因子TNF-α,IFN-γ和IL-2水平在大多
Two anti-idiotypic monoclonal antibodies 2H4 and 5D3 with nasopharyngeal carcinoma-associated antigen images were used to prepare anti-idiotypic vaccine Alum-2H4 and Alum-5D3 by aluminum hydroxide gel precipitation. Nineteen late nasopharyngeal Nine patients were treated with radiotherapy and saline injection as control group.The levels of serum antibodies and cytokines in patients before and after treatment were detected by ELISA.The levels of IL-2 mRNA in peripheral blood mononuclear cells (PBMCs) were detected by in situ Northern blotting Expression.It was found that none of the patients treated with Alum-2H4 or Alum-5D3 had anaphylactic or other side effects, anti-idiotypic antibody (Ab3) and anti-tumor antibody But also produced human anti-mouse antibody (HAMA) .The serum levels of cytokines TNF-α, IFN-γ and IL-2 levels in most