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目的:用清毒饮、养正片含药血清配合细胞因子作为培养基对急性髓系白血病完全缓解期(AML-CR)患者骨髓单个核细胞(BMNC)进行体外培养诱导树突细胞(DC),研究诱导转化的DC形态、免疫表型及分泌的细胞因子。方法:首先用清毒饮、养正片灌胃动物(新西兰兔)制备中药含药血清备用。取ALM-CR患者的骨髓,经抗凝后,应用绵羊红细胞玫瑰花结程序从骨髓中分离出T细胞的骨髓单个核细胞(TD-BMNC),以清毒饮、养正片含药血清与细胞因子联合培养,诱导含药血清促使TD-BMNC来源的DC分化成熟,观察DC的形态、免疫表型及细胞因子。结果:清毒饮+养正片组的低剂量组联合细胞因子可促进DC高表达CD1a、HLA-DR、CD80、CD86,并能促使DC高分泌细胞因子IL-2、IL-6,与对照组(细胞因子组)比较,表达均明显升高(P<0.05)。结论:清毒扶正中药可促进树突细胞的增殖和表达,提高树突细胞的抗原提呈能力。
OBJECTIVE: To induce dendritic cells (DCs) of bone marrow mononuclear cells (BMNCs) from patients with acute myeloid leukemia (AML-CR) in complete remission (AML-CR) by using Qingduyin, Yangzheng tablets containing serum and cytokines as medium, The induced DC morphology, immunophenotype, and secreted cytokines were studied. Methods: First Qingyin drink, Yangzheng tablets intragastric feeding animals (New Zealand rabbits) preparation of Chinese medicine serum containing spare. Bone marrow of patients with ALM-CR was collected. After anticoagulation, bone marrow mononuclear cells (TD-BMNCs) of T cells were isolated from the bone marrow using sheep erythrocyte rose process. Factor co-culture, inducing serum containing TD-BMNC induced differentiation and maturation of DC, observed DC morphology, immunophenotype and cytokines. Results: The low dose of Qingduyin + Yangguang group combined with cytokines could promote the expression of CD1a, HLA-DR, CD80 and CD86 in DCs and promote the secretion of IL-2 and IL-6, (Cytokine group), the expression was significantly increased (P <0.05). Conclusion: Qing Du Fuzheng Chinese medicine can promote the proliferation and expression of dendritic cells and improve the antigen presenting ability of dendritic cells.