引言组织和器官培养技术在好几年以前就已成为实验植物学的一个部分了,最近发展到考虑这些技术是否能解决林业和园艺植物的无性繁殖问题.已经试验成功的有茎尖的器官培养.目前在这个领域内的最新发展引起了很多生物工作者的注意,因它与其他学科有广泛联系.例如:原生质的分离和培养,花粉的诱导成单倍体植株.原生质体培养的重要性,在于利用 INTRODUCTION Tissue and organ culture techniques became part of experimental botany several years ago and has recently been developed to consider whether these techniques can solve the problem of vegetative propagation in forestry and horticultural plants. Successful shoot apical organ cultures have been tested. Recent developments in this area have drawn the attention of many biologists as it has extensive links with other disciplines such as the isolation and culture of protoplasts, the induction of pollen into haploid plants, the importance of protoplast culture, Is to use