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目的:建立红黄合剂的质量标准。方法:采用TLC法对红黄合剂中的大血藤、大黄、厚朴进行定性鉴别;用HPLC法测定大黄中大黄素的含量。结果:TLC特征明显,阴性对照无干扰,专属性强;大黄素在22.5～1446.0μg范围内,线性关系良好(r=0.999 7),平均加样回收率97.02%,RSD=2.16%。结论:所建立的方法能控制该药品质量。
Objective: To establish the quality standard of red and yellow mixture. Methods: TLC method was used for the qualitative identification of Rhodiola rosea, Rhubarb and Magnolia officinalis. The content of emodin in Rhubarb was determined by HPLC. Results: TLC showed obvious characteristics. The negative control had no interference and specificity. Emodin showed good linearity (r = 0.999 7) in the range of 22.5-1446.0 μg with an average recovery of 97.02% and a RSD of 2.16%. Conclusion: The established method can control the quality of the medicine.