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目的:通过人工免疫雄性大鼠获得抗精子抗体(AsAb)介导的血清AsAb阳性的免疫性不育大鼠动物模型,观察AsAb对青春期大鼠睾丸组织及睾丸生殖细胞中Fas/Fas-L凋亡途径的影响。方法:5周龄(青春期)雄性Wistar大鼠30只,其中10只处死,取精子制备精子悬液免疫大鼠,余20只动物随机分为实验组(10只)和对照组(10只),4周后摘取睾丸。光镜观察睾丸组织改变,免疫组化法检测Fas、Fas-L和Caspase-3蛋白的表达。结果:实验组睾丸组织切片呈凋亡样改变,实验组Fas、Fas-L及Caspase-3蛋白的OD值(176.97±4.58,187.52±7.76,157.65±7.38)较对照组(161.87±5.37,150.27±8.65,120.37±6.76)显著增高(P<0.01)。结论:同种精子免疫大鼠,可成功制作AsAb介导的免疫性不育模型;AsAb影响雄性大鼠的生育力,其机制可能与Fas/Fas-L凋亡途径中Fas、Fas-L和Caspase-3蛋白的表达升高有关。
OBJECTIVE: To investigate the effect of AsAb on the expression of Fas / Fas-L in testis and testicular germ cells in Astrocytes of male infertile rats induced by AsAb (AsAb) The impact of death pathway. Methods: Thirty male Wistar rats, 5 weeks old (adolescent), were sacrificed and 10 were sacrificed. Sperm were used to prepare sperm suspension immunized rats. Twenty animals were randomly divided into experimental group (n = 10) and control group (n = 10) , 4 weeks after the removal of testis. The changes of testicular tissue were observed with light microscope. The expressions of Fas, Fas-L and Caspase-3 protein were detected by immunohistochemistry. Results: The testicular tissue sections of experimental group showed apoptotic changes. The OD value of Fas, Fas-L and Caspase-3 in experimental group were significantly higher than that in control group (176.97 ± 4.58,187.52 ± 7.76,157.65 ± 7.38) (161.87 ± 5.37,150.27 ± 8.65,120.37 ± 6.76) (P <0.01). CONCLUSION: AsAb-mediated immune sterility model can be successfully made in the same kind of sperm immunization rats. AsAb can affect the fertility of male rats. The mechanism may be related to the changes of Fas, Fas-L and Caspase-3 protein expression increased.