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目的探讨泡泡浴水疗对新生期反复惊厥大鼠神经行为损伤的干预效应及其分子机制。方法出生6 d(P6)的SD大鼠36只,随机分为对照组(n=12)、惊厥组(n=12)和水疗组(n=12)。惊厥组及水疗组大鼠在P6时吸入三氟乙醚诱导惊厥发作,持续30 min,连续6 d;对照组同样操作,但不吸入三氟乙醚。水疗组于惊厥结束第2天开始进行水疗干预,为期28 d。水疗期间分时段进行负向趋地试验、悬吊试验行为学评分,水疗结束后采用Timm染色观察各组大鼠海马苔藓纤维发芽情况,荧光实时定量(RT)-PCR测定各组大鼠大脑皮质可塑性相关基因(PRG)-1 mRNA、PRG-3 mRNA的表达。结果 1.负向趋地试验评分惊厥组较对照组降低(P<0.05),经泡泡浴水疗,水疗组与对照组无显著差异;悬吊试验各组差异均无统计学意义。2.Timm染色显示,惊厥组大鼠海马齿状回内分子层和CA3区锥体细胞层可见明显异常增生苔藓纤维,水疗组异常增生苔藓纤维密度较惊厥组降低,对照组未见明显发芽。3.RT-PCR显示惊厥组大鼠大脑皮质PRG-1 mRNA、PRG-3 mRNA表达较对照组明显升高,差异均有统计学意义(Pa<0.05)。水疗组大鼠大脑皮质PRG-3 mRNA表达较惊厥组显著降低(P<0.05),PRG-1 mRNA表达虽然亦较惊厥组降低,但其差异无统计学意义(P>0.05)。结论新生期大鼠吸入三氟乙醚致反复惊厥后能够导致神经行为发育损伤,大脑皮质PRG-1、PRG-3参与发育期惊厥性脑损伤的病理生理机制,早期进行泡泡浴水疗干预对神经损伤有修复作用。
Objective To investigate the intervention effect of bubble bath spa on neurobehavioral injury in neonatal recurrent seizures and its molecular mechanism. Methods Sixty-six SD rats were randomly divided into control group (n = 12), convulsion group (n = 12) and hydrotherapy group (n = 12). Rats in convulsion group and hydrotherapy group were given trifluoroethylether for inducing seizures at P6 for 30 min continuously for 6 days. In the control group, the same procedure was performed but trifluoroacetate was not inhaled. The hydrotherapy group started hydrotherapy intervention on the second day after the convulsions ended for a period of 28 days. During the spa period, the negative trend test and the suspension test behavioral score were conducted in different periods. After the treatment, Timm staining was used to observe the sprouting of mossy fibers in the hippocampus of rats in each group. The cerebral cortex of the rats in each group was measured by real-time fluorescence quantitative PCR (RT-PCR) Plasticity related gene (PRG) -1 mRNA and PRG-3 mRNA expression. Results 1. Negative-going test scores in the seizure group were lower than those in the control group (P <0.05). There was no significant difference between the hydrotherapy group and the control group after bubble bath therapy. There was no significant difference in the suspension test between groups. 2.Timm staining showed that in the seizure group, obvious abnormal proliferation of mossy fiber was found in the dentate gyrus inner layer and CA3 pyramidal cell layer of the seizure group, and the density of dysplastic mossy fiber in the hydrotherapy group was lower than that in the seizure group and no obvious germination in the control group. RT-PCR showed that the expression of PRG-1 mRNA and PRG-3 mRNA in cerebral cortex of rats in convulsion group were significantly higher than those in control group (all P <0.05). The expression of PRG-3 mRNA in the cerebral cortex of rats in the hydrotherapy group was significantly lower than that in the convulsion group (P <0.05). The expression of PRG-1 mRNA in the hydrotherapy group was lower than that in the convulsion group (P> 0.05). Conclusions Neonatal rats inhaled trifluoroacetic acid induced recurrent seizures can lead to neurobehavioral development and injury. Cerebral cortex PRG-1 and PRG-3 are involved in the pathophysiological mechanism of development of convulsive brain injury. Early intervention of bubble bath therapy on nerve Damage repair.