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作者使用免疫磁胶系统从孕母 (孕 9～ 35周 )外周血液 (2 0 0ｍｌ)中富集ＦＴＣ与ＮＲＢＣｓ,然后应用Ｙ染色体特异性序列巢式ＰＣＲ扩增及荧光原位杂交法(ＦＩＳＨ)测定胎儿上述两种细胞 ,并以Ｘ和ＹＤＮＡ -特异性探针鉴定男胎ＦＴＣ ,同时应用带小鼠胎儿血红蛋 (ＨｂＦ)抗体
The authors used immunomagnetic system to enrich FTC and NRBCs from the peripheral blood of pregnant mothers (from 9 to 35 weeks of gestation) (200ml), and then used the Y-chromosome specific sequence nested PCR amplification and fluorescence in situ hybridization (FISH ) Were used to determine both fetal cells and male and female FTCs were identified using X and YDNA-specific probes, and antibodies against mouse fetal hemoglobin (HbF)