神经元特异性烯醇酶(NSE)是神经元和神经内分泌细胞的特异性标志酶,也是分化成熟的标志物。本实验应用HRP-SPA免疫细胞化学法,观察了生后发育中大鼠和成年猫肾上腺髓质细胞内的NSE和多巴胺-β-羟化酶(DβH)的超微结构定位。结果显示:NSE阳性颗粒定位于大鼠和猫肾上腺髓质细胞。阳性颗粒有两种类型,一种为圆形或杆形致密核心颗粒,即在深染的阳性核心周围,有一淡染的晕环,有的晕环明显,有的则不明显。另一种 Neuron-specific enolase (NSE) is a specific marker of neurons and neuroendocrine cells and is also a marker of differentiation and maturation. In this study, we used HRP-SPA immunocytochemistry to observe the ultrastructural localization of NSE and dopamine-β-hydroxylase (DβH) in adrenal medulla cells of postnatal and adult cats. The results showed that NSE positive particles localized in adrenal medulla cells of rats and cats. There are two types of positive particles, one is a round or rod-shaped core particles, that is, around the deep-stained positive core, there is a lightly stained halo, and some halo halo obvious, while others are not obvious. another