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目的:观察尼古丁对大鼠牙槽骨内碱性磷酸酶(ALP)和牙本质基质蛋白1(DMP1)C末端表达的影响。方法:将20只5周龄健康雄性Wistar大鼠随机等分为实验组和对照组,每组再随机等分为30天和60天两个亚组,实验组每天腹腔注射尼古丁0.73 mg·kg-1。分别使用钙钴法和免疫组化法检测ALP和DMP1 C末端在大鼠牙槽骨内的表达。结果:对照组:ALP和DMP1 C末端都为深棕色线形表达于牙槽骨的钙化前缘,形成明显的矿化条带;ALP和DMP1 C末端分别为棕色沉淀和棕黄色颗粒状密布于骨细胞周围。实验组:ALP和DMP1 C末端表达部位同对照组,表达强度减弱;相对于30天组,60天组变化更明显。结论:尼古丁可能通过抑制大鼠牙槽骨内ALP和DMP1 C末端的表达,抑制大鼠牙槽骨的矿化。
Objective: To observe the effect of nicotine on the C-terminal expression of alkaline phosphatase (ALP) and dentin matrix protein 1 (DMP1) in rat alveolar bone. Methods: Twenty healthy male Wistar rats of 5 weeks old were randomly divided into experimental group and control group. Each group was equally divided into two subgroups of 30 days and 60 days. The experimental group was given intraperitoneal nicotine 0.73 mg · kg -1. The expressions of ALP and DMP1 C-terminal in rat alveolar bone were detected by calcium-cobalt method and immunohistochemistry respectively. Results: In the control group, the C-terminal of ALP and DMP1 were dark brown linear expression in the calcified frontal alveolar bone, forming a clear mineralized strip; ALP and DMP1 C-terminal were brown precipitate and brown granules were densely distributed in the bone Around the cell. Experimental group: The expression of C-terminal part of ALP and DMP1 was the same as that of the control group, and the expression intensity was weakened. Compared with the 30-day group, the change of 60-day group was more obvious. Conclusion: Nicotine may inhibit the alveolar bone mineralization in rats by inhibiting the expression of C-terminal ALP and DMP1 in rat alveolar bone.