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前已报道羧甲基D-甘油醛-3-磷酸脱氢酶在紫外光照射下生成发射峰为410毫微米的新荧光物.为了探讨这一现象是否具有普遍性,我们首先研究了酵母及马肝醇脱氢酶,发现醇脱氢酶在光照下也同样能生成荧光衍生物,但生成条件与荧光物性质都与甘油醛-3-磷酸脱氢酶不同.醇脱氢酶要求较强的光照,不需要活性部位巯基的羧甲基化也不需要NAD~+的存在.所生成的荧光物的发射峰值为400毫微米.在光照过程中,醇脱氢酶迅速失活,同时320毫微米附近光吸收增加,半胱氨酸、色氨酸、酪氨酸以及组氨酸则遭到部分破坏.对十余种蛋白质及多肽类物质进行紫外光照的结果,都能生成发射峰在395—400毫微米附近的荧光衍生物,说明紫外光照产生荧光物质是蛋白质的一种普遍性质.
It has been previously reported that carboxymethyl D-glyceraldehyde-3-phosphate dehydrogenase generates a new phosphor with an emission peak of 410 nm under UV irradiation.To explore whether this phenomenon is universal, we first investigated the effects of yeast and Horse hepatic alcohol dehydrogenase, alcohol dehydrogenase found in the light can also generate fluorescent derivatives, but the conditions and properties of the phosphorescence are different with glyceraldehyde-3-phosphate dehydrogenase alcohol dehydrogenase requirements stronger Of the light does not require the active site of carboxymethyl methylation does not require the presence of NAD ~ The resulting phosphor emission peak of 400 nm in the light irradiation, alcohol dehydrogenase rapidly inactivated, while 320 Increased light absorption near the nanometer, cysteine, tryptophan, tyrosine and histidine were partially destroyed.Use of more than ten kinds of proteins and peptides UV irradiation results, can generate emission peak at Fluorescent derivatives near 395-400 nm demonstrate that UV light is a fluorescent protein that is a universal property of proteins.