本文通过溶血磷脂处理外周血淋巴细胞 (粘附细胞和非粘附细胞 )后加入维生素D3结合蛋白DBP ,37℃、 5 %CO2 孵育 3h ,其上清液超氧阴离子产物浓度明显增高 (P <0 0 1)。未用溶血磷脂处理或不加入DBP ,超氧阴离子产物无明显升高(P >0 5 )。提示溶血磷脂激活巨噬细胞 ,是通过DBP的作用而实现的。单独用溶血磷脂不能激活巨噬细胞 ,因此DBP可能是巨噬细胞激活因子前体。 In this paper, vitamin D3-binding protein DBP was added to peripheral blood lymphocytes (adherent cells and non-adherent cells) by lysophospholipid and incubated at 37 ℃ for 3h with 5% CO2. The concentration of superoxide anion in the supernatant was significantly increased (P < 0 0 1). Without lysophospholipid treatment or without adding DBP, superoxide anion product was not significantly increased (P> 0 5). Tip lysophospholipids activate macrophages, through the role of DBP achieved. Macrophages can not be activated with lysophospholipids alone, so DBP may be a precursor of macrophage activating factor.