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目的 探讨凝血酶原 (PT)分子中γ 羧基谷氨酸 (Gla)在抑制草酸钙结晶形成中的作用。 方法 采用加入不同浓度的吗啉和甲醛 (1∶10 0 0 0 )或 (1∶10 0 ) ,体外化学修饰PT ,将其分子内8个或 2个γ 羧基谷氨酸残基修饰转变成γ 亚甲基谷氨酸残基 (γ MGlu) ,草酸钙种晶抑制试验测定化学修饰后PT对草酸钙结晶抑制作用的变化。 结果 PT蛋白转变为 2γ MGlu的PT后对草酸钙结晶的抑制活性从正常对照的 14 .2降低至 12 .8(10 % ) ;PT蛋白转变为 8γ MGlu后其抑制活性降低至 5 .0 (6 5 % )。 结论 凝血酶原分子内γ 羧基谷氨酸 (Gla)在抑制草酸钙结晶形成中具有重要作用。
Objective To investigate the role of gamma-carboxyglutamic acid (GIu) in prothrombin (PT) in inhibiting the formation of calcium oxalate crystals. METHODS: PT was chemically modified in vitro by adding different concentrations of morpholine and formaldehyde (1:10 0 0) or (1:10 0) to convert 8 or 2 γ-carboxyglutamic acid residues into γMglu, calcium oxalate seed inhibition test to determine the chemical modification of PT calcium oxalate crystallization inhibition. Results After the PT protein was transformed into PT of 2γ MGlu, the inhibitory activity of PTX on the crystallization of calcium oxalate was decreased from 14.2% of the control to 12.8% (10%). After the PT protein was changed to 8γ MGlu, its inhibitory activity decreased to 5.0% 6 5%). Conclusion Prothrombin gamma-carboxyglutamic acid (GIu) plays an important role in inhibiting the formation of calcium oxalate crystals.