在体外细胞培养过程中 ,细胞生长曲线是反映细胞生物学特性十分重要的指标。传统细胞生长曲线的绘制方法 ,是通过胰酶消化后制成细胞悬液 ,显微镜下计数 ,然后计算出细胞总数[1] 。这种方法不能在不同时相或用药前后进行自身对照观察 ,因此我们在实验中采用定位微量细胞培养技 In vitro cell culture, the cell growth curve reflects the biological characteristics of cells is a very important indicator. Traditional cell growth curve mapping method is to trypsin digestion made of cell suspension, counted under a microscope, and then calculate the total number of cells [1]. This method can not be in different phases or before and after treatment for self-control observation, so we used in the experiment positioning micro-cell culture technology