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目的观察消癌解毒方对人肝癌SMMC-7721细胞相关趋化因子蛋白表达的影响,探讨消癌解毒方抗肿瘤的作用机制。方法消癌解毒方作用于人肝癌SMMC-7721细胞8 h后采用蛋白芯片技术检测药物作用前后各组相关趋化因子蛋白表达水平,采用Western blot法检测用药后各组单核细胞趋化蛋白3(MCP-3)蛋白的表达。结果与模型组比较,共有9个趋化因子蛋白下调,其中,消癌解毒方4 mg·m L-1组有3个蛋白表达下调,分别是MCP-3、Eotaxin 1、ENA 78;消癌解毒方2 mg·m L-1组有3个蛋白表达下调,分别是MCP-3、CCL28、BLC;顺铂组共有6个蛋白表达下调,分别是CXCL16、ENA 78、Eotaxin 1、XCL1、MIP-3 beta、MPIF 1。Western blot结果显示,与模型组比较,消癌解毒方4 mg·m L-1组、2 mg·m L-1组MCP-3蛋白均出现下调,顺铂组无明显差异。结论消癌解毒方可能是通过影响趋化因子信号通路中的某些蛋白来发挥抗肿瘤的作用。
Objective To observe the effect of Xiaoyao Jiedu Fang on chemokine protein expression in human hepatocellular carcinoma SMMC-7721 cells and explore its anti-tumor mechanism. Methods Xiaojian Jiedu Fang on human hepatocellular carcinoma SMMC-7721 cells 8 h after the use of protein chip technology to detect the drug before and after each group of relevant chemokine protein expression levels by Western blot assay after treatment monocyte chemotactic protein 3 (MCP-3) protein expression. Results Compared with the model group, a total of 9 chemokines were downregulated. Among them, 3 protein expression was down-regulated in the 4 mg · m L-1 group, namely MCP-3, Eotaxin 1 and ENA 78; The expression of 3 proteins in detoxification 2 mg · m L-1 group was down-regulated, which were MCP-3, CCL28 and BLC respectively. A total of 6 proteins were down-regulated in CXCL16, ENA 78, Eotaxin 1, XCL1, MIP -3 beta, MPIF 1. Western blot results showed that compared with the model group, MCP-3 protein in 4 mg · m L-1 and 2 mg · m L-1 groups of Xiaogui Jiedu Decoction were all decreased, but there was no significant difference in cisplatin group. Conclusion Xiaojian Jiedu Fang may play an anti-tumor role by affecting certain proteins in the chemokine signaling pathway.