The present paper mainly studies the petroleum system of the Sufyan Depression in the Muglad Basin of central Africa and analyzes its control of hydrocarbon accumulation. On the basis of comprehensive analysis of effective source rock, reservoir bed types and source-reservoir-seal assemblages, petroleum system theory has been used to classify the petroleum system of the Sufyan Depression. Vertically, the Sufyan Depression consists of two subsystems. One is an Abu Gabra subsystem as a self generating, accumulating and sealing assemblage. The other subsystem is composed of an Abu Gabra source rock, Bentiu channel sandstone reservoir and Darfur group shale seal, which is a prolific assemblage in this area. Laterally, the Sufyan Depression is divided into eastern and western parts with separate hydrocarbon generation centers more than 10 000 m deep. The potential of the petroleum system is tremendous. Recently, there has been a great breakthrough in exploration. The Sufyan C-1 well drilled in the central structural belt obtained high-yielding oil flow exceeding 100 tons per day and controlled geologic reserves of tens of millions of tons. The total resource potential of the Sufyan Depression is considerable. The central structural belt is most favorable as an exploration and development prospect. The present paper mainly studies the petroleum system of the Sufyan Depression in the Muglad Basin of central Africa and analyzes its control of hydrocarbon accumulation. On the basis of comprehensive analysis of effective source rock, reservoir bed types and source-reservoir-seal assemblages, petroleum system theory has been used to classify the petroleum system of the Sufyan Depression. Vertically, the Sufyan Depression consists of two subsystems. One is an Abu Gabra subsystem as a self-generating, accumulating and sealing assemblage. The other subsystem is composed of an Abu Gabra source rock, Bentiu channel sandstone reservoir and Darfur group shale seal, which is a prolific assemblage in this area. Laterally, the Sufyan Depression is divided into eastern and western parts with separate hydrocarbon generation centers more than 10 000 m deep. The potential of the petroleum system is tremendous. Recently, there has been a great breakthrough in exploration. The Sufyan C-1 well drilled in the central structural belt obtained high-yielding oil flow exceeding 100 tons per day and controlled geologic reserves of tens of millions of tons. The total resource potential of the Sufyan Depression is considerable. The central structural belt is most favorable as an exploration and development prospect.