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Cohlan发现,过量维生素A(Vit A)可使大鼠胎发生畸形;Rosa报道,孕妇在妊娠初期,服用过量Vit A,可导致自然流产或胎儿畸形,但对Vit A致畸机理至今不明。Padmanabhan曾对大鼠胎眼发生及过量Vit A所致眼畸形做了研究,本文应用组织化学和电镜技术,以期对Vit A致畸机理加以探讨。一、材料和方法用未经产雌性Wistar大鼠,体重200～250g,普通饲料及饮水喂养。雌鼠和雄鼠2:1合笼过夜,次日7～9时,作阴道涂片检查,查到精子者定为受精的第0d。Vit A(Sigma
Cohlan found that excessive vitamin A (Vit A) can cause fetal deformity in rats; Rosa reported that pregnant women in the early pregnancy, taking excessive Vit A, can lead to spontaneous abortion or fetal malformations, but the mechanism of Vit A teratogenicity is unknown. Padmanabhan has done studies on the occurrence of fetal eye defects and visual abnormalities caused by excessive vitamin A. In this paper, histochemistry and electron microscopy were used to investigate the mechanism of Vit A teratogenesis. First, materials and methods Unproductive female Wistar rats, weighing 200 ~ 250g, ordinary feed and drinking water. Female and male rats 2: 1 cage overnight, 7 to 9 the next day for vaginal smear, sperm found to be fertilized 0d. Vit A (Sigma