重金属镉(Cd)作为一种非生命活动所需的常见污染物,在土壤中以低浓度存在时,便可以对生物体产生极强的毒性,影响农作物的生长发育.为了解大豆对重金属Cd胁迫应答的分子机制,把发芽7 d的大豆苗在75μmol·L~(-1)Cd浓度中处理0、4、8、12和48 h,然后取根进行转录组表达分析.共得到2670个表达差异基因,其中4、8、12和48 h处理组中分别有244、1545、442和1401个基因显示了表达差异.这些基因通过GO分类,可分为56类;采用COG数据库进行比对,根据其功能大致可分为25类.KEGG通路富集分析表明,差异表达基因主要富集在苯丙氨酸代谢、泛醌和其他萜类醌生物合成,以及半胱氨酸和甲硫氨酸代谢等通路中.发现3个异黄酮2’-羟化酶基因、2个异黄酮还原酶基因和1个查尔酮合成酶基因在Cd胁迫下均表达上调.RT-PCR检测4个差异表达基因的表达模式与RNA-Seq分析结果一致,证实了RNA-Seq结果的可靠性. Heavy metal cadmium (Cd) as a non-living activities required for common pollutants in the soil at low concentrations, it can produce very strong toxicity to the organism, affecting crop growth and development.In order to understand the effects of soybean on heavy metal Cd The molecular mechanism of stress response was that the germinated soybean seedlings at 7 d were treated for 0, 4, 8, 12 and 48 h at a concentration of 75 μmol·L -1 Cd, and then rooted for transcriptome expression analysis. The difference of gene expression was found among the 4, 8, 12 and 48 h treatment groups, and there were 244, 1545, 442 and 1401 genes in the treatment group respectively.The expression of these genes was classified into 56 categories according to the GO classification.Comparison with the COG database , According to its function can be divided into 25 categories.KEGG pathway enrichment analysis showed that the differentially expressed genes are mainly concentrated in phenylalanine metabolism, ubiquinone and other terpene quinone biosynthesis, as well as cysteine ​​and methionine Acid metabolism and other pathways found three isoflavone 2’-hydroxylase gene, two isoflavone reductase gene and a chalcone synthase gene were upregulated under Cd stress.RT-PCR detection of four differences The expression pattern of the expressed genes was in agreement with the results of RNA-Seq analysis and confirmed that the results of RNA-Seq reliability.