论文部分内容阅读
以中熟桃‘京玉’和‘大久保’果实为材料,测定桃果实内果皮在细胞分裂和硬核期糖的含量,分析糖积累的变化情况;通过间苯三酚—HCl对‘大久保’桃果实内果皮进行染色,观察木质素沉积的进程;应用常规戊二醛—锇酸双固定法在透射电镜下观察内果皮细胞壁的发育进程。结果表明,桃果实内果皮在花后40d前,细胞不断分裂增生,初生细胞壁不断膨大,糖的积累快速增长,大约40d时葡萄糖和果糖的积累量达到最高;到40d后,糖含量及山梨醇代谢迅速下降。此时内果皮开始木质化,内部逐渐呈现红色,大约半个月后,整个内果皮都变红,木质素从内到外基本沉积完毕,说明木质素沉积发生在糖积累高峰之后,糖的大量积累为木质素前体的合成奠定了物质基础,糖积累量的多少直接影响内果皮发育的好坏。
The mid-ripe peach ’Jingyu’ and ’Okubo’ fruits were used as materials to determine the content of endocarp peach in the cell division and the hard-core sugar, and the changes of sugar accumulation were analyzed. By using phloroglucinol-HCl, Peach fruit endocarp was dyed to observe the progress of lignin deposition. The development of endocarp cell wall was observed under transmission electron microscope by conventional glutaraldehyde-osmic acid double fixation method. The results showed that 40 days after anthesis, the cells continued to divide and proliferate, the primary cell wall expanded and the accumulation of sugar increased rapidly. The accumulation of glucose and fructose reached the highest level after about 40 days. After 40 days, the content of sugar and sorbitol Metabolism rapidly declines. At this moment, the endocarp began to be lignified, and the interior gradually showed red color. After about half a month, the entire inner pericarp became reddish. The lignin basically deposited from the inside to the outside, indicating that lignin deposition occurred after the peak of sugar accumulation. Accumulation of lignin precursor synthesis laid the material foundation, the amount of sugar accumulation directly affects the quality of endocarp development.