论文部分内容阅读
研究了meso 四 (对羟基苯基 )卟啉为柱前衍生化试剂与Cd2 +,Pb2 +,Cu2 +和Zn2 +离子的配合反应条件及配合物在C1 8色谱柱上的分离条件 ,建立了反相高效液相色谱快速分离光度检测Cd2 +,Pb2 +,Cu2 +和Zn2 +的新方法。配合物和试剂在 1 5min内出峰完毕。 4种离子的检出限为 :Cd2 +0 0 2ng ,Pb2 +0 0 2ng ,Cu2 +0 0 2ng ,Zn2 +0 1 2ng ;线性范围为 :Cd2 +0 8μg/L～ 1 50 μg/L ,Pb2 +0 8μg/L～ 30 0 μg/L ,Cu2 +0 8μg/L～ 50 0 μg/L ,Zn2 +5 0 μg/L～ 1 0 0 0 μg/L ;方法的日内相对标准偏差为 :2 8%～ 4 8%,测定低、中、高 3个浓度的日间相对标准偏差为 3 7%～ 9 7%。
The conditions for the complexation of meso tetra (p-hydroxyphenyl) porphyrin with the pre-column derivatization reagent and the Cd2 +, Pb2 +, Cu2 + and Zn2 + ions and the separation conditions of the complexes on the C1 8 column were studied. Rapid Determination of Cd2 +, Pb2 +, Cu2 + and Zn2 + by Reversed Phase High Performance Liquid Chromatography. The complexes and reagents peaked within 15 min. The detection limits of the four ions were: Cd2 +0 0 2ng, Pb2 +0 0 2ng, Cu2 +0 0 2ng, Zn2 +0 1 2ng; the linear range was Cd2 +0 8μg / L ~ 1 50μg / Pb2 +0 8 μg / L ~ 30 0 μg / L, Cu2 +0 8 μg / L ~ 50 0 μg / L and Zn2 +5 0 μg / L ~ 100 μg / L. The relative standard deviations 28% ~ 48%. The relative standard deviations (RSDs) of three low, middle and high concentrations were 37% ~ 97%.