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以尾巨桉优良无性系无菌苗茎段为外植体,通过对噻唑基脲类新型分裂素(N-phenyl-N′-[6-(2-chlorobenzothiazol)-yl]urea,PBU)等多种不同浓度生长调节剂组合的优化,进行胚性愈伤组织诱导及植株再生研究。结果表明,在添加了2mg·L-1PBU和0.05mg·L-1IAA的改良MS培养基上,茎段外植体培养5d后愈伤组织诱导率达96%以上。将愈伤组织接种在添加1mg·L-16-BA和0.05mg·L-1NAA的MS培养基上,诱芽率达90.8%。随后在添加了0.8mg·L-1PBU与0.05mg·L-1IAA的1/2MS培养基上诱导芽伸长,用1/2MS培养基附加0.5mg·L-1IBA诱导生根,移栽后得到完整再生植株。
Using the stem of aseptic seedlings of Eucalyptus urophylla × Eucalyptus as explant, the effects of N-phenyl-N’- [6- (2-chlorobenzothiazol) -ylidene] urea and PBU Optimization of a combination of growth regulators at different concentrations for embryogenic callus induction and plant regeneration. The results showed that the callus induction rate reached more than 96% on the MS medium supplemented with 2mg · L-1 PBU and 0.05mg · L-1IAA after 5 days. The calli were inoculated on MS medium supplemented with 1 mg · L-16-BA and 0.05 mg · L-1 NAA, and the bud-inducing rate was 90.8%. Subsequently, shoot elongation was induced on 1/2 MS medium supplemented with 0.8 mg · L-1 PBU and 0.05 mg · L-1 IAA, and rooting was induced with 1/2 MS medium supplemented with 0.5 mg · L -1 IBA, resulting in complete after transplanting Regenerate plants.