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怀山药脱毒试管苗经诱导可形成脱毒微型块茎,其作为繁殖体比试管苗具有优势。以继代培养的脱毒试管苗带芽茎段为外植体,接种于含60 g·L-1蔗糖的MS液体培养基中,采用黑暗震荡的培养方式进行微型块茎诱导,分别在外植体时期、出芽期、芽伸长期、微型块茎形成期、膨大期和成熟期取材进行相关生理生化指标的测定。结果表明,微型块茎形成过程中可溶性总糖、葡萄糖和果糖含量变化趋势基本一致,均在微型块茎形成期达最大值;蔗糖和淀粉逐渐积累,含量逐步升高;蔗糖合成酶(SS)合成方向活性与蔗糖磷酸合成酶(SPS)活性分别在微型块茎成熟期和膨大期达最大值,共同促进微型块茎内蔗糖的积累;腺苷二磷酸葡萄糖焦磷酸化酶(AGPase)和淀粉合成酶(SSS)分别在酶活性最高时与淀粉合成积累量呈极显著相关;IAA、ABA和ZR含量均与微型块茎形成正相关,JA与微型块茎形成无明显相关。
The yam drug-free test-tube seedlings can induce the formation of virus-free microtuber, which has advantages over the test-tube seedlings as the propagule. The explants were cultured in MS liquid medium containing 60 g · L -1 sucrose by subculturing the detoxified in vitro test-tube shoots with buds and stem segments. The explants were induced by dark shaking culture, Period, budding stage, elongation stage budding, mini tuber formation, swelling and maturity of the material for the determination of the relevant physiological and biochemical indicators. The results showed that the content of total soluble sugar, glucose and fructose remained the same during the formation of mini tubers, and reached the maximum during the formation of mini tubers. The sucrose and starch gradually accumulated and the content gradually increased. The synthesis direction of sucrose synthase Activity and sucrose phosphate synthase (SPS) activity reached the maximum value at the ripening stage and the expanding stage of microtuber, respectively, and promoted the accumulation of sucrose in mini tuber; AGPase and starch synthase (SSS) ) Were significantly and significantly correlated with the accumulation of starch synthesis at the highest enzyme activity. IAA, ABA and ZR contents were positively correlated with microtuber formation, while JA was not significantly correlated with microtuber formation.