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采用农杆菌介导的方法将水稻Na+/H+反向转运器基因OsNHX1导入84K杨,获得3株抗性转化植株,PCR,Southern和Northern检测结果表明,OsNHX1基因已经整合到84K杨基因组中,并可以稳定表达.耐盐实验表明,3个株系的转基因植株能在200mmol/LNaCl条件下正常生长.对盐胁迫处理的转基因植株进行Na+含量和渗透势测定,发现转基因植株叶片中的Na+明显高于对照植株,其渗透势明显低于对照植株.分子检测和耐盐性实验表明OsNHX1基因的转化获得成功,并获得84K杨耐盐转基因植株.
Agrobacterium-mediated transformation of rice Na + / H + antiporter OsNHX1 into 84K poplar resulted in 3 resistant transformed plants. The results of PCR, Southern and Northern analyzes showed that the OsNHX1 gene was integrated into the genome of 84K The results of salt-tolerance experiments showed that the three transgenic plants could grow normally under the condition of 200mmol / L NaCl.Na + content and osmotic potential of the transgenic plants treated with salt stress were significantly higher than those in the transgenic plants Compared with the control plants, the osmotic potential of control plants was significantly lower than that of the control plants, and the molecular tests and salt tolerance experiments showed that the transformation of OsNHX1 gene was successful and 84K Yang salt tolerant transgenic plants were obtained.