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本文电泳调查了日本1767野生大豆个体的种子胰蛋白酶抑制剂类型及频率分布。日本野生大豆存在两类型,即Tia和Tib,频率分别为94.5％和5.5％。从纬度上看,34°～38°带比其它纬度带有较高的Tib频率。本调查看到约1％的材料是Tia/Tib杂和体。 首次观察了3种蛋白的变异性。这些蛋白具有明显的多态性及地理分布差异。K1有3个形态:缺失体,快带和慢带;K2有两种形态:快带和慢带;B有两种形态:快带和慢带。本文提出这3种蛋白可以做为“等位酶”,用于野生大豆地理遗传分化的研究。 日本野生大豆的种皮过氧化物酶活性表型频率略高于中国,荧光型频率远高于中国。初步讨论了日本天然野生大豆群体的遗传变异,似乎由低纬度到高纬度地理变异的程度增大。
In this paper, the electrophoresis of Japanese 1767 wild soybean seed trypsin inhibitor type and frequency distribution. There are two types of wild Japanese soybeans, Tia and Tib, with frequencies of 94.5% and 5.5%, respectively. In terms of latitude, the 34 ° ~ 38 ° band has a higher Tib frequency than other latitudes. About 1% of the material in this survey was Tia / Tib hybrids. For the first time observed the variability of three proteins. These proteins have obvious polymorphism and geographical distribution differences. K1 has three forms: missing body, fast and slow belt; K2 has two forms: fast and slow belt; B has two forms: fast and slow belt. This paper proposes that these three proteins can be used as “allozymes” for the study of geographical genetic differentiation of wild soybeans. Japanese wild soybean pericarp peroxidase activity phenotype frequency slightly higher than China, the fluorescence frequency is much higher than China. Preliminary discussion of the genetic variation in Japanese natural wild soybean populations seems to have increased from low latitudes to high latitudes.