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目的调查近1年武汉大学人民医院住院的手术前、输血前、介入前以及辅助生殖技术前患者血清梅毒螺旋体(Treponema pallidum,TP)抗体的检测情况,对梅毒的疾病监测数据进行分析。方法用甲苯胺红不加热血清试验(Toluidine Red Untreated Serum Test,TRUST)和化学发光微粒子免疫检测法(Chemiluminescence microparticle immunoassay,CMIA)分别检测TP非特异性抗体和特异性抗体。结果手术前、输血前、介入前和辅助生殖技术前患者TP特异性抗体阳性率分别为1.54%,1.40%,1.79%和1.44%,TP非特异性抗体阳性率分别为0.28%,0.23%,0.11%,0.41%。统计学分析发现,男、女组TP特异性抗体和非特异性抗体阳性率差异均无统计学意义(P>0.05);而不同年龄组间TP特异性抗体阳性率差异有统计学意义(P<0.05),其中男性组>60岁患者TP抗体阳性率最高,女性组年龄41~50岁患者TP抗体阳性率最高。结论手术前、输血前、介入前及辅助生殖技术前患者的TP检出率均较高,且与年龄有关。有必要对手术前、输血前、介入前及辅助生殖技术前患者进行TP检测。
Objective To investigate the detection of serum anti-Treponema pallidum (TP) antibodies in pre-transfusion, pre-transfusion and pre-assisted reproductive technologies in Wuhan People’s Hospital in the past year and analyze the surveillance data of syphilis. Methods TP nonspecific antibodies and specific antibodies were detected by Toluidine Red Untreated Serum Test (TRUST) and Chemiluminescence microparticle immunoassay (CMIA). Results Before operation, the positive rates of TP-specific antibodies before transfusion, before intervention and before assisted reproductive technology were 1.54%, 1.40%, 1.79% and 1.44% respectively. The positive rates of TP non-specific antibodies were 0.28%, 0.23% and 0.11 %, 0.41%. Statistical analysis showed that there was no significant difference in the positive rates of TP-specific antibodies and nonspecific antibodies between male and female groups (P> 0.05), while there was a significant difference in the positive rate of TP-specific antibodies between different age groups (P < 0.05). The positive rate of TP antibody in male patients> 60 years old was the highest. The positive rate of TP antibody in women aged 41-50 years was the highest. Conclusions Before operation, the detection rate of TP before transfusion, before intervention and before assisted reproductive technique were both high and age-related. There is a need for TP testing before surgery, before transfusion, before intervention and before assisted reproductive technology.