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目的探讨共刺激分子OX40/OX40L在过敏性紫癜(HSP)患儿外周血单个核细胞(PBMC)中的表达及其在HSP发病机制及治疗中的作用及意义。方法选取首次住院HSP患儿34例(HSP组),另取健康体检儿童20例作为健康对照组。分别采集外周静脉血5 mL,用密度梯度离心法制备新鲜PBMC,分为对照培养组;植物血凝素(PHA)刺激组;PHA+OX40L单克隆抗体(Anti-OX40LmAb)组。培养48 h后分别抽取RNA,应用反转录-PCR(RT-PCR)从转录水平检测PBMC中OX40 mRNA和OX40L mRNA的表达。结果在对照培养组中,HSP组OX40 mRNA、OX40LmRNA的表达均明显高于健康对照组(Pa<0.01)。加入PHA刺激后,2组OX40 mRNA、OX40LmRNA表达均明显增高(Pa<0.01),且HSP组较健康对照组明显增高(Pa<0.01);加入Anti-OX40LmAb后2组OX40 mRNA、OX40LmRNA表达均明显下降(Pa<0.01)。结论 HSP患儿PBMC共刺激分子OX40和OX40L的mRNA表达异常增高,OX40/OX40L这一对共刺激分子可能参与了HSP的发病及发展,阻断该共刺激通路有望为HSP的治疗提供新思路。
Objective To investigate the expression of costimulatory molecule OX40 / OX40L in peripheral blood mononuclear cells (PBMCs) of children with Henoch-Schonlein Purpura (HSP) and its role in the pathogenesis and treatment of HSP. Methods Forty-three HSP patients were recruited from hospital for the first time and 20 healthy children were selected as healthy control group. 5 mL of peripheral venous blood was collected respectively. Fresh PBMCs were prepared by density gradient centrifugation and divided into control group, PHA-stimulated group and PHA + OX40L monoclonal antibody group. After cultured for 48 h, RNA was extracted and the expression of OX40 mRNA and OX40L mRNA in PBMC was detected by RT-PCR at the transcriptional level. Results In control group, the expressions of OX40 mRNA and OX40L mRNA in HSP group were significantly higher than those in healthy control group (Pa0.01). After adding PHA, the expression of OX40 mRNA and OX40L mRNA in both groups were significantly increased (Pa <0.01), and the HSP group was significantly higher than that in healthy control group (Pa <0.01). The expression of OX40 mRNA and OX40L mRNA in two groups were significantly increased after adding Anti-OX40LmAb Decreased (Pa <0.01). Conclusion The mRNA expression of costimulatory molecules OX40 and OX40L in PBMCs of HSP patients is abnormally elevated. OX40 / OX40L costimulatory molecule may be involved in the pathogenesis and development of HSP. Blocking the costimulatory pathway may provide new ideas for the treatment of HSP.