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目的优化、完善分析植物性食物中5种类黄酮成分的方法。方法采用Nova-Pak C18(3.9 mm×150mm,4μm)色谱柱;流动相为0.1%三氟乙酸(A)-5%乙腈甲醇溶液(B),梯度洗脱(0～5min,90%A→60%A;5～18 min,60%A→40%A;18～21 min,40%A→10%A;21～25 min,10%A→10%A;25～30 min,10%A→90%A),流速1 ml/min;检测波长365 nm,柱温35℃。结果杨梅黄酮、槲皮素、玉米黄酮、坎二菲醇、芹菜配基5种类黄酮在0.156～20μg/ml浓度范围内与色谱峰高呈良好的线性关系,相关系数(r)为0.9995～0.9999;检测限为0.0195μg/ml(S/N≥3);低、中、高浓度的平均加标回收率(n=3)为88.5%～105.6%,相对标准偏差(RSD)均小于6.32%。结论该方法灵敏、准确,具有良好的重复性和稳定性,可用于植物性食物中5种类黄酮含量的检测。
Objective To optimize and perfect the method for the analysis of five flavonoids in plant foods. Methods A Nova-Pak C18 column (3.9 mm × 150 mm, 4 μm) was used. The mobile phase consisted of 0.1% trifluoroacetic acid (A) and 5% acetonitrile in methanol 60% A to 40% A; 18 to 21 minutes, 40% A to 10% A; 21 to 25 minutes, 10% A to 10% A; 25 to 30 minutes, 10% A → 90% A), flow rate 1 ml / min; detection wavelength 365 nm, column temperature 35 ℃. Results The flavonoids of myricetin, quercetin, zeaxanthin, canndifen and celery ligand had a good linear relationship with the chromatographic peak height in the range of 0.156 ~ 20μg / ml with the correlation coefficient (r) of 0.9995 ~ 0.9999 The detection limit was 0.0195μg / ml (S / N≥3). The average recoveries (n = 3) were 88.5% -105.6% at low, middle and high concentrations with RSDs less than 6.32% . Conclusion The method is sensitive, accurate, reproducible and stable and can be used for the determination of five flavonoids in plant foods.