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在高粱同核异质雌性不育系(3197A、T×398A)和异核异质雌性不育系(FA)、保持系的花药及雌蕊不同发育时期,共检测出13种正、负极向过氧化物酶同工酶。两种同核异质雄性不育系的过氧化物酶谱型非常接近,因此,这种酶可能是该基因编码的。 在3197A、T×398A中检测出2条专一性酶带(A_8、A_9),这两条输带可能是同核异质雄性不育系的标志酶带。所有三套雄性不育系花粉粒中的过氧化物酶同工酶匀多于保持系,这可能是雄性不育细胞质基因对核基因表达的调控作用所造成。细胞质基因与细胞核基因存在着一定的相互关系,雄性不育特性可能是这两种基因共同作用的结果。
Thirteen kinds of positive and negative were detected in different developmental stages of anther and pistil in sorghum with nuclear allogeneic female sterile line (3197A, T × 398A) and heterogenous nuclear heterogeneous female sterile line (FA) Oxidase isoenzyme. The peroxidase profiles of the two isoforms of heteronuclear male sterility are very close and therefore this enzyme may be encoded by this gene. Two specific bands (A_8, A_9) were detected in 3197A and T × 398A. These two bands may be the sign bands of homonuclear allogenic male sterile lines. The peroxidase isoenzyme in pollen grains of all three male sterile lines was more uniform than the maintainer line, which may be caused by the regulatory effect of male sterility cytoplasm on nuclear gene expression. Cytoplasmic genes and nuclear genes exist a certain relationship, male sterility may be the result of the interaction of these two genes.