Conventional organic solar cell’s(OSC) architectures, including rigid transparent substrate(Glass), conductive electrode(Indium tin oxide, ITO) and small working areas, are widely utilized in organic photovoltaic fields. However, such a structure as well as conventional spin-coating method obviously restrict their industrial application. In this article, we report the deposition of silver nanowires(Ag NWs) on the flexible substrate by slot-die printing. The obtained Ag NWs films exhibited a high transmittance and a low resistance, and were further used as the transparent conductive electrode of OSCs. A typical conjugated polymer, poly[(2,5-bis(2-hexyldecyloxy)phenylene)-alt-(5,6-difluoro-4,7-di(thiophen-2-yl)benzo[c] [1,2,5]thiadiazole)](PPDT2FBT), was used as the active material to fabricate large-area(7 cm~2) solar cells by a slot-die coating process. The power conversion efficiency(PCE) could reach 1.87% initially and further increased to 3.04% by thermal annealing. Compared to the performance of reference cell on ITO substrate, the result indicated that the Ag NWs could be developed as an alternative substitute of conductive electrode to fabricate the large-area flexible OSCs by roll-to-roll printing. Conventional organic solar cell’s (OSC) architectures, including rigid transparent substrates (Glass), conductive electrodes (Indium tin oxide, ITO) and small working areas, are widely utilized in organic photovoltaic fields. However, coating method obviously restrict their industrial application. In this article, we report the deposition of silver nanowires (Ag NWs) on the flexible substrate by slot-die printing. The obtained Ag NWs films show a high transmittance and a low resistance, and even further used as the transparent conductive electrode of OSCs. A typical conjugated polymer, poly [(2,5-bis (2-hexyldecyloxy) phenylene) -alt- (5,6-difluoro-4,7-di ) benzo [c] [1,2,5] thiadiazole]] (PPDT2FBT), was used as the active material to fabricate large-area (7 cm ~ 2) solar cells by a slot-die coating process. (PCE) could reach 1.87% initially and yet increased to 3.04% by thermal annealing. Compared to the performance of reference cell on ITO substrate, the result indicated that the Ag NWs could be developed as an alternative substitute of conductive electrode to fabricate the large-area flexible OSCs by roll-to-roll printing.