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A variety of genes involved in nodule development and function were induced during sym biotic root nodule development in alfalfa. We identi fied those genes by comparing the cDNA-amplified fragment length polymorphism (cDNA-AFLP) pat terns of infected nodules and uninfected roots. Sev enty-one transcript-derived fragments (TDFs) were isolated from about 3000 TDFs, which are enhanced or specifically expressed in nodule. The differentia expression patterns of 61 genes were confirmed b reverse Northern hybridization or Northern hybridiza tion analyses. Among these, 31 exhibited significan similarities to characterized database entries. These results suggest that the genes corresponding to the 31 TDFs may play important roles in signal transduc tion, gene expression and regulation, substance transportation or auto-regulation of nodulation during nodule development. The Northern hybridization analysis also indicates that the gene corresponding to TDF RX89 was not expressed in the nodule elic ited by bacA mutant, presumably it is specially in duced at the stage of nodule development when bacteroids begin to be differentiated.
A variety of genes involved in nodule development and function were induced during sym biotic root nodule development in alfalfa. We identi fied those genes by comparing the cDNA-amplified fragment length polymorphism (cDNA-AFLP) pat terns of infected nodules and uninfected roots. Sev The differentia expression patterns of 61 genes were confirmed in reverse Northern hybridization or Northern hybridization analyzes. Among these, 31 exhibited several TDFs, which were enhanced or expressed in nodules. significan similarities to characterized database entries. These results suggest that the genes corresponding to the 31 TDFs may play important roles in signal transduc tion, gene expression and regulation, substance transportation or auto-regulation of nodulation during nodule development. The Northern hybridization analysis also indicates that the gene corresponding to TDF RX89 was not expressed in the nodule eli c ited by bacA mutant, presumably it is specially in duced at the stage of nodule development when bacteroids begin to be differentiated.