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目的 探求在大鼠肝硬化形成的早期 ,红细胞电泳率和红细胞膜胆固醇含量的变化情况。方法 将Wistar大鼠随机分成对照组和肝硬化组 ,其中肝硬化组又分成 2、5、7、10周组 ,每组 10只。肝组织行H E和天狼猩红染色 ,下腔静脉取血检测红细胞电泳率和红细胞膜胆固醇的含量。结果 在本实验中 ,肝硬化 7w鼠的肝组织中有大量的胶原纤维增生并开始形成假小叶。对照组和 2w肝硬化组间的红细胞电泳率的差异不明显 (P >0 .0 5 ) ,而自给药的第 5周起出现明显的增加 ;肝硬化 2w鼠红细胞膜胆固醇含量明显升高 ,至第 5周开始降至正常水平以下。结论 本大鼠肝硬化模型的红细胞电泳率和红细胞膜胆固醇含量在第 5周后出现明显的异常 ,比形态学上出现假小叶等典型的肝硬化表现约提前了 2周时间 ,为临床肝硬化的早期诊断治疗提供实验依据。
Objective To explore the changes of erythrocyte electrophoresis rate and erythrocyte membrane cholesterol content in the early stage of cirrhosis of rats. Methods Wistar rats were randomly divided into control group and cirrhosis group. The cirrhosis group was divided into 2, 5, 7 and 10 weeks group with 10 in each group. H E and Sirius red staining were performed in the liver tissue, and the erythrocyte electrophoresis rate and erythrocyte membrane cholesterol content in the inferior vena cava were measured. Results In this experiment, a large number of collagen fibers proliferated and began to form pseudolobules in hepatic tissues of cirrhotic rats for 7 weeks. There was no significant difference in erythrocyte electrophoretic rate between the control group and the 2-week cirrhosis group (P> 0.05), but a significant increase appeared from the 5th week of the administration; the erythrocyte membrane cholesterol content increased significantly in the 2-week cirrhotic rats, It started to fall below the normal level by week 5. CONCLUSION: The erythrocyte electrophoresis rate and erythrocyte membrane cholesterol content in rat cirrhotic rat model showed obvious abnormalities after 5 weeks, which was about 2 weeks earlier than the typical cirrhosis such as pseudolobule morphology, The early diagnosis and treatment to provide experimental evidence.