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本研究探讨4种白血病和淋巴瘤细胞系以及非肿瘤细胞系和正常人骨髓Id4基因启动子甲基化状态及其差异。采用甲基化特异性聚合酶链反应(MS-PCR)对白血病细胞系K562和HL-60、淋巴瘤细胞系Ramous和CA46以及良性细胞系Hek937和正常人骨髓细胞进行Id4基因甲基化状态检测。结果表明:Id4基因在正常人骨髓细胞和Hek937细胞系中呈完全非甲基化状态,在4个血液肿瘤细胞系K562、HL-60、Ramous和CA46中均呈甲基化状态。结论:所检测的4种血液肿瘤细胞系中,Id4基因的甲基化状态与正常人骨髓细胞和非肿瘤细胞系完全不同,Id4基因甲基化模式的改变与造血细胞恶变的发生密切相关。
This study was to investigate the promoter methylation status of Id4 gene in four kinds of leukemia and lymphoma cell lines, non-tumor cell lines and normal human bone marrow and their differences. The methylation status of Id4 gene was detected by methylation-specific polymerase chain reaction (MS-PCR) in leukemia cell lines K562 and HL-60, lymphoma cell lines Ramous and CA46, benign cell line Hek937 and normal human bone marrow cells . The results showed that Id4 gene was completely unmethylated in normal human bone marrow cells and Hek937 cells, and methylated in all four hematological tumor cell lines, K562, HL-60, Ramous and CA46. CONCLUSION: The methylation status of Id4 gene is completely different from normal human bone marrow cells and non-tumor cell lines in the 4 hematological tumor cell lines tested. The change of methylation pattern of Id4 gene is closely related to the malignant transformation of hematopoietic cells.