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在呼吸相关神经元或其它任何类型的神经元中,与生理性自发活动相对应的电突触电流(gap junction currents,GJCs)尚未在单个神经元中被记录到,因此电突触如何参与呼吸相关的或其它类型的生理性活动,目前所知甚少。在本研究中,我们假设GJCs可在电压钳记录条件下通过消除跨膜电化学梯度在单个神经元实现选择性记录,并在单个吸气性气管迷走神经节前神经元(inspiratory tracheal preganglionic vagal motor neurons,I-TPVMs)进行验证。结果显示,用这种方法在所有I-TPVMs中均记录到GJCs,且这些神经元的GJCs可被节律性中枢吸气活动所激活。此法可用于快速探测具自发活动的神经元网络中的GJCs。
In respiratory-related neurons or any other type of neurons, gap junction currents (GJCs), which correspond to physiological spontaneous activity, have not been recorded in a single neuron, so how electrical synapses participate in respiration Associated or other types of physiological activity, little known at the moment. In the present study, we hypothesized that GJCs could selectively record in a single neuron by eliminating the transmembrane electrochemical gradient under voltage clamp recording conditions, and that a single inspiratory tracheal preganglionic vagal motor neurons , I-TPVMs). The results showed that GJCs were recorded in all I-TPVMs by this method, and GJCs of these neurons were activated by rhythmic central inspiratory activity. This method can be used to rapidly probe GJCs in spontaneous neuronal networks.