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目的探讨DNA结合抑制因子4(Id4)基因甲基化与急性白血病(AL)的关系。方法采用甲基化特异性(MS)-PCR对正常人骨髓、外周血、外周血干细胞(PBSC),以及AL细胞系和AL患者的骨髓进行Id4基因启动子区甲基化状况分析。结果。Id4基因在正常骨髓、外周血和PBSC中呈完全性非甲基化状态。25例初治急性髓系白血病中有21例Id4基因甲基化,甲基化比例84％。14例初治急性淋巴细胞白血病中有12例Id4基因甲基化,甲基化比例达86％。8例复发和难治的AL患者该基因均呈甲基化。在K562和HL60细胞系中均呈完全甲基化,在Hek937细胞系则呈完全非甲基化。结论与正常人不同,在AL患者及白血病细胞系中,Id4基因启动子区都发生了高程度的甲基化改变,Id4基因甲基化模式的改变与AL的发生密切相关。
Objective To investigate the relationship between methylation of DNA binding inhibitor 4 (Id4) gene and acute leukemia (AL). Methods Methylation specificity (MS) -PCR was used to analyze the promoter methylation status of Id4 gene in bone marrow of normal people, peripheral blood, peripheral blood stem cells (PBSC), and AL and AL cell lines. result. The Id4 gene is completely unmethylated in normal bone marrow, peripheral blood and PBSC. Twenty-one of the 25 newly diagnosed acute myeloid leukemia patients had methylation of Id4 gene and the methylation rate was 84%. In 14 cases of newly diagnosed acute lymphoblastic leukemia, 12 cases of Id4 gene methylation, methylation rate of 86%. The gene was methylated in 8 relapsed and refractory AL patients. It was completely methylated in both K562 and HL60 cell lines and completely unmethylated in Hek937 cell line. Conclusion Different from normal people, there is a high degree of methylation change in the promoter region of Id4 gene in AL patients and leukemia cell lines. The methylation pattern of Id4 gene is closely related to the occurrence of AL.